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活性蛋白 > 其他 > Recombinant Human Novel Coronavirus Spike glycoprotein(S), partial
  • 产品名称:

    Recombinant Human Novel Coronavirus Spike glycoprotein(S), partial IN STOCK

  • 货期:
    3-7 工作日
  • uniprot号:
  • 货号:
    CSB-YP3324GMY1
  • 规格:
  • 图像:


    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    Predicted band size: 38.2 kDa

    Observed band size:

    (a) 68 kDa before EndoH Digestion

    (b) 38 kDa after EndoH Digestion



    Activity

    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.



    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml.



    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml.



    SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 pM as detected by LSPR Assay.


  • 测活实验手册:
  • 基因名称:
    S,S1-RBD
  • 种属:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 蛋白描述:
    Partial
  • 分子量:
    38.2 kDa
  • 标签信息:
    N-terminal 6xHis-sumostar-tagged
  • 来源:
    Yeast
  • 纯度:
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性:
    ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml.③Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml.④SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 pM as detected by LSPR Assay.
  • 氨基酸序列:
    RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLN
    DLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK
    SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPK
    KSTNLVKNKCVNF
  • 说明书:

    注:产品说明书可能有改进,请直接与CUSABIO产品专员联系,索取最新版说明书!

  • 蛋白储存:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
  • 表达区域:
    319-541aa
  • 使用注意:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 冻干前缓冲:
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 状态:
    Lyophilized powder
  • 货期:
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 研究领域:
    Microbiology