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抗体 > 重组抗体 > CD9 Antibody
  • 产品类型:
    Recombinant Antibody
  • 产品名称:

    CD9 Antibody

  • 英文名称:

    CD9 Antibody

  • 货号:
    CSB-RA004969A0HU
  • 规格:
    ¥2,000
  • 别名:
    Tetraspanin 29 antibody; 5H9 antibody; 5H9 antigen antibody; Antigen defined by monoclonal antibody 602 29 antibody; Antigen defined by monoclonal antibody 60229 antibody; BA-2/p24 antigen antibody; BA2 antibody; BTCC 1 antibody; BTCC1 antibody; CD9 antibody; CD9 antigen antibody; CD9 antigen p24 antibody; CD9 molecule antibody; CD9_HUMAN antibody; Cell growth inhibiting gene 2 protein antibody; Cell growth-inhibiting gene 2 protein antibody; DRAP 27 antibody; DRAP27 antibody; GIG2 antibody; Growth inhibiting gene 2 protein antibody; Leukocyte antigen MIC3 antibody; MIC3 antibody; Motility related protein antibody; Motility-related protein antibody; MRP 1 antibody; MRP-1 antibody; MRP1 antibody; p24 antibody; p24 antigen antibody; Tetraspanin-29 antibody; Tspan 29 antibody; Tspan-29 antibody; TSPAN29 antibody
  • 种属:
    Homo sapiens (Human)
  • 免疫原:
    A synthesized peptide
  • 种属反应性:
    Human
  • 应用范围:
    ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:30-1:200
  • 背景:
    Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • 克隆类型:
    Monoclonal
  • 抗体亚型:
    Rabbit IgG
  • 纯化方式:
    Affinity-chromatography
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:

    Western Blot

    Positive WB detected in:U87 whole cell lysate

    All lanes:CD9 antibody at 0.55μg/ml

    Secondary

    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 25 KDa

    Observed band size: 25 KDa

    IHC image of CSB-RA004969A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

    Immunofluorescence staining of MCF-7 cells with CSB-RA004969A0HU at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

    Overlay histogram showing Jurkat cells stained with CSB-RA004969A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

  • 说明书:

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  • Accession No.:
  • 指标名称:
    CD9
  • 研究领域:
    Cardiovascular