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抗体 > 多克隆抗体 > NUP62 Antibody
  • 产品类型:
    Polyclonal Antibody
  • 产品名称:

    NUP62 Antibody

  • 货号:
    CSB-PA016204LA01HU
  • 规格:
    ¥1200
  • 别名:
    NUP62 antibody; Nuclear pore glycoprotein p62 antibody; 62 kDa nucleoporin antibody; Nucleoporin Nup62 antibody
  • 种属:
    Homo sapiens (Human)
  • 免疫原:
    Recombinant Human Nuclear pore glycoprotein p62 protein (173-522AA)
  • 宿主:
    Rabbit
  • 种属反应性:
    Human, Mouse
  • 应用范围:
    ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000
  • 背景:
    Essential component of the nuclear pore complex (PubMed:1915414). The N-terminal is probably involved in nucleocytoplasmic transport (PubMed:1915414). The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed:1915414, PubMed:24107630). Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed:24107630). It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed:24107630).
  • 克隆类型:
    Polyclonal
  • 抗体亚型:
    IgG
  • 纯化方式:
    >95%, Protein G purified
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:

    Western Blot

    Positive WB detected in: Jurkat whole cell lysate, RAW264.7 whole cell lysate, PC-3 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysate, A549 whole cell lysate

    All lanes: NUP62 antibody at 3.5μg/ml

    Secondary

    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 54 kDa

    Observed band size: 62 kDa

    IHC image of CSB-PA016204LA01HU diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

    Immunoprecipitating NUP62 in A549 whole cell lysate

    Lane 1: Rabbit control IgG instead of CSB-PA016204LA01HU in A549 whole cell lysate.
    For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)

    Lane 2: CSB-PA016204LA01HU (6μg) + A549 whole cell lysate (1mg)

    Lane 3: A549 whole cell lysate (20μg)

  • 说明书:
  • Accession No.:
  • 指标名称:
    NUP62
  • 研究领域:
    Epigenetics and Nuclear Signaling; Cancer; Cell biology; Tags & Cell Markers; Signal transduction