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抗体 > 多克隆抗体 > HIST1H2BB Antibody
  • 产品类型:
    Polyclonal Antibody
  • 产品名称:

    HIST1H2BB Antibody

  • 货号:
    CSB-PA010402HA01HU
  • 规格:
    ¥1200
  • 别名:
    HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody
  • 种属:
    Homo sapiens (Human)
  • 免疫原:
    Recombinant Human Histone H2B type 1-B protein (2-126AA)
  • 宿主:
    Rabbit
  • 种属反应性:
    Human, Mouse, Rat
  • 应用范围:
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:500
  • 背景:
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 克隆类型:
    Polyclonal
  • 抗体亚型:
    IgG
  • 纯化方式:
    >95%, Protein G purified
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:


    Western Blot

    Positive WB detected in: Mouse liver tissue, Rat liver tissue

    All lanes: HIST1H2BB antibody at 2.8μg/ml

    Secondary

    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 14 kDa

    Observed band size: 14 kDa



    IHC image of CSB-PA010402HA01HU diluted at 1:800 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    IHC image of CSB-PA010402HA01HU diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    Immunofluorescent analysis of PC-3 cells using CSB-PA010402HA01HU at dilution of 1:266 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • 说明书:
  • Accession No.:
  • 指标名称:
    HIST1H2BB
  • 研究领域:
    Others