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抗体 > 多克隆抗体 > EMD Antibody
  • 产品类型:
    Polyclonal Antibody
  • 产品名称:

    EMD Antibody

  • 货号:
    CSB-PA007633LA01HU
  • 规格:
    ¥1200
  • 别名:
    EDMD antibody; Emd antibody; EMD_HUMAN antibody; Emerin antibody; Emery Dreifuss muscular dystrophy antibody; STA antibody
  • 种属:
    Homo sapiens (Human)
  • 免疫原:
    Recombinant Human Emerin protein (1-222AA)
  • 宿主:
    Rabbit
  • 种属反应性:
    Human, Mouse
  • 应用范围:
    ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
  • 背景:
    Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
  • 克隆类型:
    Polyclonal
  • 抗体亚型:
    IgG
  • 纯化方式:
    >95%, Protein G purified
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:


    Western Blot

    Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Colo320 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Mouse lung tissue

    All lanes: EMD antibody at 2µg/ml

    Secondary

    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 29 kDa

    Observed band size: 35 kDa



    Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA007633LA01HU at dilution of 1:100



    Immunohistochemistry of paraffin-embedded human bladder cancer using CSB-PA007633LA01HU at dilution of 1:100



    Immunofluorescent analysis of Hela cells using CSB-PA007633LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)



    Immunoprecipitating EMD in Hela whole cell lysate

    Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA007633LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

    Lane 2: CSB-PA007633LA01HU (8µg) + Hela whole cell lysate (500µg)

    Lane 3: Hela whole cell lysate (10µg)

  • 说明书:
  • Accession No.:
  • 指标名称:
    EMD
  • 研究领域:
    Signal Transduction