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试剂盒 > 酶联免疫试剂盒 > 人骨桥素(OPN)ELISA Kit
    • ELISA Kit
    • 人骨桥素(OPN)ELISA Kit
    • A segmental duplication of the p arm of chromosome 16 created two identical copies of the GIY-YIG domain containing gene, this record represents the more centromeric copy. Exons of this gene overlap with exons of the phenol-preferring sulfotransferase (SULT1A3) gene. Two transcript variants that encode different protein isoforms have been identified through sequence analysis.[provided by RefSeq]
    • UniProt
    • BIOWWW
    • HGNC
    • RGD
    • MGI1921572
    • OMIM609150
    • CUSABIOCSB-E08392h
  • 产品类型:
    ELISA Kit
  • 产品名称:

    人骨桥素(OPN)ELISA Kit

  • 英文名称:

    Human Osteopontin,OPN ELISA Kit

  • 货号:
    CSB-E08392h
  • 别名:
    BNSP, BSPI, ETA-1, MGC110940, OPN, SPP1/CALPHA1 fusion|bone sialoprotein I|early T-lymphocyte activation 1|osteopontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|s
  • 规格:
    96T
  • 中文价格:
    3200
  • 种属:
    Homo sapiens (Human)
  • 其他种属:
    Canis lupus familiaris (Dog) (Canis familiaris) Equus caballus (Horse) Odocoileus virginianus virginianus (Virginia white-tailed deer) Pan troglodytes (Chimpanzee) 吉利彩票投注Pan paniscus (Pygmy chimpanzee) (Bonobo) Bos taurus (Bovine) Saimiri boliviensis boliviensis (Bolivian squirrel monkey) Capra hircus (Goat) Cavia porcellus (Guinea pig) Canis familiaris (Dog) (Canis lupus familiaris) Oryctolagus cuniculus (Rabbit) Sciurus carolinensis (Gray squirrel) Phodopus sungorus (Striped hairy-footed hamster) (Djungarian hamster) Rattus norvegicus (Rat) Podarcis sicula (Italian wall lizard) Homo sapiens (Human) Felis catus (Cat) (Felis silvestris catus) Rutilus rutilus (Roach) Mus musculus (Mouse) Xenopus laevis (African clawed frog) Danio rerio (Zebrafish) (Brachydanio rerio) Gadus morhua (Atlantic cod) Branchiostoma belcheri (Amphioxus)
  • 待测物名称:
    secreted phosphoprotein 1
  • 缩写:
    OPN
  • 蛋白功能1:
    Tumor marker
  • 蛋白功能3:
    Biomineralization
  • 检测范围:
    0.78 ng/mL-50 ng/mL
  • 灵敏度:
    0.195 ng/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.418 2.333 2.376 2.271
    251.684 1.706 1.695 1.590
    12.51.175 1.152 1.164 1.059
    6.250.654 0.664 0.659 0.554
    3.120.383 0.361 0.372 0.267
    1.560.245 0.232 0.239 0.134
    0.780.176 0.185 0.181 0.076
    00.102 0.108 0.105  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human OPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:5Average %94
    Range %88-98
    1:10Average %100
    Range %94-104
    1:20Average %93
    Range %88-97
    1:40Average %99
    Range %93-104
  • 回收率:
    The recovery of human OPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9581-97
    EDTA plasma (n=4)10297-106
  • 定性/定量:
    quantitative
  • 研究领域:
    Cancer
  • 引用文献:
    1. , Wei QS et al,Orthop Surg,2017 Feb;7(1):57-65.
    2. , Motegi SI. et al,Acta Derm Venereol.,2015 Nov 4;95(8):978-84.
    3. , Wei QS. et al,Scand J Clin Lab Invest,2015 Sep 28:1-7.
    4. , AA Mehde et al,Journal of Advances in Management Research ,2014; 7(1):1169-1174
    5. , Fouad SA. et al,World J Gastroenterol,2015,24(4):135-145
    6. , Bo Li.et al,Materials Chemistry B,2016,(4):683-693
    7. , Manaka S.et al,FEBS Lett.,2015,589(3):310-8.
    8. , Robati RM. et al,Int J Dermatol,2016 Jan 15.
    9. , DongWang.et al,Life Sciences,July 9
    10. , Mehmet Arif Icer .et al,Clinical Biochemistry,August 13