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试剂盒 > 酶联免疫试剂盒 > Guinea pig insulin (INS) ELISA kit
    • ELISA Kit
    • Guinea pig insulin (INS) ELISA kit
    • After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region.[provided by RefSeq]
    • UniProt
    • HGNC
    • RGD
    • MGI1347487
    • OMIM602341
    • CUSABIOCSB-EL011742GU
  • 产品类型:
    ELISA Kit
  • 产品名称:

    Guinea pig insulin (INS) ELISA kit

  • 英文名称:

    Guinea pig insulin (INS) ELISA kit

  • 货号:
    CSB-EL011742GU
  • 别名:
    INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
  • 规格:
    96T
  • 中文价格:
    4200
  • 种属:
    Guinea Sus scrofa (Pig)
  • 其他种属:
  • 待测物名称:
    insulin
  • 缩写:
    INS
  • 蛋白功能1:
    Biosynthesis/Metabolism
  • 蛋白功能2:
    glyconeogenesis and glycometabolism
  • 蛋白功能3:
    Carbohydrate metabolism
  • 检测范围:
    12 μIU/mL-210 μIU/mL
  • 灵敏度:
    7.5 μIU/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    12 0.049 0.051 0.050    
    24 0.165 0.160 0.163    
    48 0.377 0.374 0.376    
    120 0.853 0.849 0.851    
    210 1.686 1.691 1.689    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-97  
    1:2 Average % 96  
    Range % 90-106  
    1:4 Average % 95  
    Range % 86-104  
    1:8 Average % 90  
    Range % 87-94  
  • 回收率:
    The recovery of guinea pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-101  
    EDTA plasma (n=4) 95 89-103  
                 
                 
  • 定性/定量:
    quantitative
  • 研究领域:
    Metabolism
  • 引用文献:
    1. , Jiang Q, et al,Archives of Animal Nutrition,2019
    2. , KaiguoGao,Animal Feed Science and Technology,2019