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试剂盒 > 酶联免疫试剂盒 > 吉利彩票投注牛皮质醇(Cortisol)ELISA Kit
    • ELISA Kit
    • 牛皮质醇(Cortisol)ELISA Kit
  • 产品类型:
    ELISA Kit
  • 产品名称:

    牛皮质醇(Cortisol)ELISA Kit

  • 英文名称:

    Bovine Cortisol ELISA Kit

  • 货号:
    CSB-E13064B
  • 规格:
    96T
  • 中文价格:
    4200元
  • 种属:
    Bos taurus (Bovine)
  • 其他种属:
  • 待测物名称:
    Cortisol
  • 缩写:
    COR
  • 蛋白功能1:
    Metabolism
  • 检测范围:
    0.049 ng/mL-200 ng/mL
  • 灵敏度:
    0.049 ng/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    200 0.194 0.184 0.189    
    50 0.328 0.320 0.324    
    12.5 0.507 0.550 0.529    
    3.12 0.900 0.975 0.938    
    0.78 1.314 1.266 1.290    
    0.195 1.653 1.657 1.655    
    0.049 1.915 1.902 1.909    
    0 2.306 2.400 2.353    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-99  
    1:2 Average % 96  
    Range % 92-105  
    1:4 Average % 85  
    Range % 81-89  
    1:8 Average % 91  
    Range % 83-98  
  • 回收率:
    The recovery of bovine cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-95  
    EDTA plasma (n=4) 96 92-102  
                 
                 
  • 定性/定量:
    quantitative
  • 研究领域:
    Metabolism
  • 引用文献:
    1. , Ovais Aarif. et al,Theriogenology,2015 Oct 15;84(7):1197-205.
    2. , Cheng N. et al,Metab Brain Dis.,2015 Aug;30(4):1087-91.
    3. , WY Park. et al,Journal of Animal Production Advances,2015; 5(10):765-770.
    4. , Park W. Y. et al,Journal of Animal Production Advances,2015,5(11): 788-793
    5. , Park W. Y.et al,Journal of Animal Production Advances,2015,5(11): 820-826
    6. , Aarif O.et al,Theriogenology.,84(7):1197-205
    7. , Alhussien, Mohanned Naif.et al,biological rhythm research ,13 Jul
    8. , Mohanned NaifAlhussien.et al,Veterinary Immunology and Immunopathology,29 July
    9. , Preeti Lakhani.et al,Biological Rhythm Research ,Jan 12
    10. , Mohanned Naif Alhussien.et al,Theriogenology,Mar 29
    11. , Chun-Nam Cha.et al,J. Prev. Vet. Med,April
    12. , Alhussien MN.et al,Vet Immunol Immunopathol,Sep
    13. , Mohanned NaifAlhussien.et al,Microbial Pathogenesis,May 19
    14. , Mohanned Naif Alhussien.et al,Journal of Thermal Biology,August 25
    15. , Park.et al,Korea Journal of Organic Agriculture,August
    16. , Lakhani N.et al,tropical animal health and production,Octobert 17
    17. , Seckin SALAR, et al,Turkish Journal of Veterinary and Animal Sciences,May 7