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试剂盒 > 酶联免疫试剂盒 > 牛皮质醇(Cortisol)ELISA Kit
    • ELISA Kit
    • 牛皮质醇(Cortisol)ELISA Kit
  • 产品类型:
    ELISA Kit
  • 产品名称:

    牛皮质醇(Cortisol)ELISA Kit

  • 英文名称:

    Bovine Cortisol ELISA Kit

  • 货号:
    CSB-E13064B
  • 规格:
    96T
  • 中文价格:
    4200元
  • 种属:
    Bos taurus (Bovine)
  • 其他种属:
  • 待测物名称:
    Cortisol
  • 缩写:
    COR
  • 蛋白功能1:
    Metabolism
  • 检测范围:
    0.049 ng/mL-200 ng/mL
  • 灵敏度:
    0.049 ng/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    200 0.194 0.184 0.189    
    50 0.328 0.320 0.324    
    12.5 0.507 0.550 0.529    
    3.12 0.900 0.975 0.938    
    0.78 1.314 1.266 1.290    
    0.195 1.653 1.657 1.655    
    0.049 1.915 1.902 1.909    
    0 2.306 2.400 2.353    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-99  
    1:2 Average % 96  
    Range % 92-105  
    1:4 Average % 85  
    Range % 81-89  
    1:8 Average % 91  
    Range % 83-98  
  • 回收率:
    The recovery of bovine cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-95  
    EDTA plasma (n=4) 96 92-102  
                 
                 
  • 定性/定量:
    quantitative
  • 研究领域:
    Metabolism
  • 引用文献:
    1. , EK Park,Veterinarski arhiv,2020
    2. , Jiseon Son,Animal Science,2020
    3. , Colakoglu H E, et al,Domestic Animal Endocrinology,2019
    4. , Seckin SALAR, et al,Turkish Journal of Veterinary and Animal Sciences,2019
    5. , Lakhani N.et al,tropical animal health and production,2018
    6. , Park.et al,Korea Journal of Organic Agriculture,2018
    7. , Mohanned Naif Alhussien.et al,Journal of Thermal Biology,2018
    8. , Mohanned NaifAlhussien.et al,Microbial Pathogenesis,2018
    9. , Chun-Nam Cha.et al,J. Prev. Vet. Med,2018
    10. , Alhussien MN.et al,Vet Immunol Immunopathol,2017
    11. , Mohanned Naif Alhussien.et al,Theriogenology,2018
    12. , Preeti Lakhani.et al,Biological Rhythm Research ,2018
    13. , Mohanned NaifAlhussien.et al,Veterinary Immunology and Immunopathology,2017
    14. , Alhussien, Mohanned Naif.et al,biological rhythm research ,2017
    15. , Aarif O.et al,Theriogenology.,2015
    16. , Park W. Y.et al,Journal of Animal Production Advances,2015
    17. , Park W. Y. et al,Journal of Animal Production Advances,2015
    18. , WY Park. et al,Journal of Animal Production Advances,2015
    19. , Cheng N. et al,Metab Brain Dis.,2015
    20. , Ovais Aarif. et al,Theriogenology,2015